In this study, we have measured developmental and sex-based differences in hypothalamic kisspeptin expression and distribution in the hpg mouse model, to address questions of the programming role of the HPG axis itself on the kisspeptinergic system.
We consider that our adherence, competence and overall impression measures are developmental, and that in the future the use of triangulated data from multiple sources and more differentiated, contextually sensitive measures specifically designed for complex interventions may prove to be of great value.
In the following, we refer to the array experiments measuring developmental stages and tissues as YALE-1, while the array describing expression data for stress conditions is termed YALE-2.
We measure developmental variation by age, and distinguish those aged 35 and under from those aged 36 and over.
This difference could be due to the parameters measured (developmental delay vs. cell and wing size) or to the remnants of hppy function in the alleles used in each experiment.
Awasthi 2000 measured developmental status using the Denver Questionnaire, and did not demonstrate an effect of deworming.
To measure developmental speed we compared stage-matched brains ex vivo and in vivo.
Data on infant outcomes were only collected for up to 4 months, reducing the ability of the registry to measure developmental progress, defects diagnosed beyond 4 months of age, and resolution of suspected defects reported in early infancy.
Both the Mental Developmental Index (MDI) and Psychomotor Developmental Index (PDI) were used to measure developmental outcomes.
Further differences may arise from the fact that Hes1 is highly regulated both transcriptionally and post-transcriptionally; oscillations in Hes1 protein levels play a critical role in measuring developmental time in unsegmented paraxial mesoderm [21].
Finally, data on infant outcomes were only collected for up to 4 months, reducing the ability of the registry to measure developmental progress.
