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This technique measures copy number variations (CNVs) within the entire genome of a disease sample compared to a normal sample [ 11].
Array-based comparative genomic hybridization (aCGH) measures copy number variations at multiple loci simultaneously, providing an important tool for studying genomic alterations associated with cancer, developmental disorders, and germline copy number polymorphisms.
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However, multiplex PCR cannot quantitatively measure copy number of genomic DNA.
For CNV, the large number of replicates provides sufficient precision to accurately measure copy number states.
Copy number profiles can be inferred along with point mutations from sequencing data of sufficient depth [ 14], and the use of assays such as SNP arrays that measure copy number but not point mutations may thus become less frequent as sequencing costs decrease.
CGH measures DNA copy number differences between a test and reference genome.
CGH differs from Reverse Transcription Polymerase Chain Reaction (RT-PCR) in that it measures DNA copy number variations rather than messenger RNA expression.
To measure DNA copy number in the RH cell lines, we used array comparative genomic hybridization (aCGH) of each clone compared to the reference hamster A23 recipient line.
Microarray comparative genomic hybridization (CGH) is currently one of the most powerful techniques to measure DNA copy number in large genomes.
Although recent developments have enabled experiments to measure copy number on a genome scale with high genomic resolution, individual point measurements are still noisy, making the crucial separation of signal from noise difficult.
Circulating plasma mitochondrial DNA (mt-DNA) were assessed by measuring copy number of the NADH dehydrogenase 1 gene using quantitative real-time PCR.
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