Thus it was not possible to measure cellularity, cellular activity, biochemical properties and vascularity of tendon.
Cells were transfected with siRNA, and the number of viable cells at the indicated time points was determined using Cell Counting Kit-8, which measures cellular dehydrogenase activity.
Mycolactone present in ASL from tissues were quantified using the MTT assay, a colorimetric test that measures cellular metabolic activity of human embryonic lung fibroblasts (HELF) [ 11, 15, 17] as previously described.
Phosphorylation of ERK and CREB can be induced by several different cellular pathways [23], [24], [25] and so analyses of these proteins provides a coarse measure of cellular activity.
For example, assays of biological activity to measure cellular uptake, activity inhibition, or cytotoxicity of a conjugate, are commonly employed to demonstrate clinical advantages of multivalent conjugates.
Several assay systems are available to measure the cellular activities of Gα proteins.
We measured cellular metabolic activity (Fig. 2C) of MDA-MB-231 cells stably expressing hSulf2 and found decreased metabolic activity and reduced proliferation without change in cell viability (Fig. 2E) compared to control cells.
The outcomes of this study can be easily extended to the signaling of neural networks such as cultured primary neurons or brain slices, where it is necessary to measure long-term cellular activity in a large working area [43, 44].
The MTT colori-metric assay can be used to measure changes in cellular activity and cell viability.
The changes in the cell antioxidant (SOD, GPx) and oxidant (H2O2) enzyme activities were measured using respective cellular activity assay kits (Jiancheng Biochemical Co., Ltd., Nanjing, China).
