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This assay measures cell wall recalcitrance by quantifying the amount of monomeric sugars released during cell wall digestion [ 1- 5].
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In order to test the efficiency of this method for quantifying anaerobic rumen fungi, it has been attempted to evaluate this method in in vitro conditions by comparing with an assay based on measuring cell wall chitin.
However this are not measuring cell wall anisotropy in-plane with these experiments as the text seems to claim.
Cell wall thickness was measured directly from the CLSM images by a procedure adapted from those used previously to measure cell wall thickness in wood [ 16, 17].
To examine the effects of the artificial microRNA constructs on cell wall structure, we measured cell wall thickness in the toluidine blue-stained sections.
Chitin levels were determined by staining hyphae with CFW and by measuring cell wall chitin content by high-pressure liquid chromatography (HPLC).
Nanoindentation is a method used for measuring the mechanical properties of thin films and surface layers, which is believed to be applicable for measuring the cell wall mechanical properties of Al-foams as well.
In this paper, the nanoindentation technique has been used for measuring the cell wall mechanical properties of Al Si Cu Mg alloy foams.
This method gave a reliable measure for cell wall localized poly P, but the actual content might be underestimated, since a part of cell wall bound poly P chains might be inaccessible for degradation by ScPpx1.
The values for insoluble and soluble fractions were obtained by the Englyst procedure, which aims to measure plant cell wall non-starch polysaccharides as the sum of the chemically identified constituent sugars; the procedure does not measure lignin (Englyst et al, 1994).
Here we have explored the potential use of alamethicin for permeabilization of A. thaliana Columbia (Col-0) and tobacco BY-2 cells to measure synthesis of cell wall polymers.
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