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As shown in the Figure 3B and Figure S1, cycE siRNA significantly reduced the ability of SK-BR3 to grow on soft agar, a well known assay that measures cell transformation.
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Cell transformation was measured by using a soft agar colony forming assay as described previously.
One method of analysing the tumorigenic potential is the soft agar assay, which measures anchorage-independent growth, which is an indicator for cell transformation.
Cells were trypsinized and tested for soft agar colony formation using CytoSelect 96-Well Cell Transformation Assay (Cell Biolabs), using the CyQuant GR dye to measure total cellular nucleic acid levels.
inhibitor saturation constant of type I type II cell transformation.
Moreover, overexpression of CIP2A promotes human cell transformation [20].
The resumed proliferative capacity diverts Ras activity to cell transformation.
AC performed the tobacco leaf cell transformation.
The significant of spontaneous transformation in in vitro rat cell transformation systems is discussed.
It can inhibit cell transformation and activity of cancer gene.
Hotspot mutations of Ras drive cell transformation and tumorigenesis.
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