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First, after lysis of red blood cells (RBCs), the tungsten-halogen based optical system of the MPO channel measures cell size by forward light scatter, and stain intensity by absorbance, thereby counting and differentiating granulocytes, lymphocytes, and monocytes based on their size and MPO content.
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Using chemical stains they developed to measure cell size and the number of nuclei, the investigators determined that the heart expansion was from hypertrophy, not formation of new cells.
At regular intervals after elutriation, we measured cell size and the fraction of budded cells.
To quantify these differences, we measured cell size with a fluorescence-activated cell sorter (FACS); the forward-angle light scatter (FSC) parameter correlates with cell diameter [7].
To investigate this, we measured cell size across the studied cell lines using the forward scattering channel of the flow cytometer.
To measure cell size in parallel on a genome-wide scale, we subjected pools of the haploid deletion collection grown in rich media to centrifugal elutriation.
We analyzed these images using an automated analysis program that identified cells expressing DsRed, determined if they were myocytes based on the intensity of anti-α-actinin staining, and then measured cell size.
This is further investigated by measuring cell size.
For measuring cell size at septation, cells without cdr2-GFP were analyzed.
EPCs images were used to measure cell size with ImageJ (http://rsb.info.nih.gov/ij/).
Wheat germ agglutinin (WGA) staining was carried out to measure cell size.
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