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Cells were thawed and treated with CyQuant GR dye, which binds stoichiometrically to nucleic acids and, thus, measures cell number.
pxy wox14 lines were identical to pxy single mutants for all parameters tested, i.e. for vascular proliferation measures (cell number in inflorescence stem vascular bundles and hypocotyl diameter; supplementary material Figs S4, S5) and for vascular organisation phenotypes (Analine Blue stained sections; supplementary material Fig. S5).
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In addition, for MCF-7 cells we verified the utility of this assay for measuring cell number by comparison with DNA content measurements and with cell counts by hemocytometer (data not shown).
Cells were treated with nine concentrations of drug for 72 h before measuring cell number relative to controls [12].
Resazurin, a soluble dye that is reduced to highly fluorescent resorufin in proportion to the metabolic activity of a cell population, is a valuable, noninvasive tool to measure cell number.
For therapeutic antibodies that inhibit the growth of cancer cells, proliferation assays that measure cell number changes after the antibody treatment are often used to determine the potency of the antibody.
Another important difference is that Kreider et al [32] measured cell number and size by the DNA concentration and content while we used immunohistochemical staining method in our experiments.
We first measured cell number after serial dilution of plated cells to verify the acquisition.
We used flow cytometry to measure cell number and individual cells' fluorescence.
We first checked for defects in cell proliferation by measuring cell number in Drosophila S2 cells upon RNAi-mediated depletion of genes of interest.
The utility of the CV assay in measuring cell number was verified both in MCF-7 cells and in combination with immunodetection plate assays for GH3/B6 cells [ 17].
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