Instead of counting cells by hand or measuring cell density, we believe that measurement of biochemical markers in the supernatant or in homogenates of the slices will provide a more feasible readout of such an assay.
Commercially-available, light emission-based bioassays were selected for measuring cell density, activity, and diversity.
Ten micro-liters of the culture medium was divided and transferred onto a hemocytometer (Marienfeld, Germany) to measure cell density.
Levitation is far from the only method for measuring cell density, notes Grover, who has developed a different technique for weighing single living cells by sticking them onto a tiny resonator and measuring changes in its vibration.
Immediately before inoculation we measured cell density and cell biovolume in each stock culture to estimate algal biomass [44].
The 0.5 ml aliquot was used to measure cell density at 600 nm using an Eppendorf BioPhotometer.
We incubated PAB076 in YPD, 2x YPD, SDC and 2x SDC media and measured cell density over time.
The cell suspension was analyzed with a Guava EasyCyte base system from Guava Technologies (Hayward, California, US) to accurately measure cell density and cell viability via ViaCount kit.
Cell growth was determined by measuring cell density (in cells/mL) and dry cell weight (DCW).
Planktonic subpopulations were measured by removing nonadhering cells by pipetting and measuring cell density in new microtiter wells.
