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Dye solution absorbance was measured before and during dyeing using a 2550 UV Vis Spectrophotometer (Shimadzu, Japan) and measurements were evaluated using 'Vision 32' software.
The solubility measurements were evaluated using a statistical experimental design.
Differences in time for filling out the TiC-P at baseline and during follow-up measurements were evaluated using a paired sample t-test.
Statistical differences between two groups in qRT-PCR, immunohistochemistry, Northern blot, RNA immunoprecipitation experiments and in plasma osmolality and AVP measurements were evaluated using unpaired Student's t-test.
Serial measurements were evaluated using the means of subsequent measurements as described [ 14], and these means of measurements were compared with baseline values by Wilcoxon paired test.
The linear range, limits of detection and reproducibility of microarray and RT-qPCR measurements were evaluated using panels of RNA standards.
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The reliability of the measurements was evaluated using paired samples t test.
The time period for short-time measurements is evaluated using stochastic simulations.
Intra- and inter-observer reproducibility of these measurements was evaluated using intra-class correlation coefficients.
The efficiency of the measurements was evaluated using reverse extraction of standard fluoride solution at a concentration of 0.1 and 1 ppm.
Differences between measurement conditions were evaluated using t tests.
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