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Mean values and standard deviations (SDs) for flexural strength measurements were calculated using the Minitab 16 program for Windows 8 (Minitab, State College, PA, USA).
The error can be estimated to be typically ∼10%; statistic errors of single measurements were calculated using the method of Harrison and Hazell (SIA, 18, 1992, p. 368 376).
A representative sequence from each OTU was assigned a taxonomic classification based on the Ribosomal Database Project's naïve Bayesian classifier (Wang et al.2007) at an 80% confidence threshold; and all richness and diversity measurements were calculated using the mothur software package based on a random subsampling subset of 1958 sequences to equal the number of reads in the smallest library.
All image-based measurements were calculated using the COMSTAT biofilm image processing package in Matlab [22].
Colorimetric measurements were calculated using a Synergy HT multi-Detection microplate reader with Gen5 software (Biotek Instruments, Winooski, VT).
Area measurements were calculated using Scion Image Scion Corporationn).
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The average from each of these measurements was calculated using Zetasizer series software 6.20 (Malvern Instrument).
The method error of measurements was calculated using Dahlberg's formula [15]: Method error = √ ∑ d 2 / 2 n.
Agreement between these measurements was calculated using Spearman's correlation.
The precision of each gravity measurement was calculated using a least-squares method incorporating a model of solid-Earth tides to remove linear drifts.
Percentage error to determine acceptable limits of agreement between both techniques of cardiac index measurement was calculated using the formula given by Critchley and Critchley [21].
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