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Behavioural data, MRI volumes and T2 SI measurements were analysed using repeated measures (RM) two-way analysis of variance (ANOVA) with lesion and time as main effects.
Measurements were analysed using repeated measures ANOVA.
Femoral angle measurements were analysed using a mixed effects linear model for repeated measures to determine the levels of intra-observer agreement (repeatability) and inter-observer agreement (reproducibility).
Radioactivity (ratios to non-target tissues and autoradiography) measurements were analysed using a two-way analysis of variance.
The experimental results of conductivity measurements were analysed using the Lee—Wheaton equation.
These measurements were analysed using MORPHOJ [ 69].
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The cross-sectional association between each exposure and FEV1, defined as the best of three satisfactory measurements, was analysed using multiple linear regression controlling for sex, age, age squared, smoking status, pack-years of cigarettes smoked, height, Carstairs deprivation score and age-height interaction.
Longitudinal measures were analysed using repeated measurements ANOVA.
For all mucus, histology and immunostaining measurements data were analysed using a two-tailed Mann–Whitney U-test.
For comparison of differences between means at specific dates and initial (0 day) measurements, data were analysed using a Student's t-test at the 0.05 or 0.01 probability level following an F-test of equality of variances.
Measurement data were analysed using SPSS V.13.0 (SPSS, Chicago, Illinois, USA) for statistical analysis and expressed in mean±SD.
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