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We measured nearly identical proximity ratios for the Ndc80 complex measurements using this FRET pair (Fig. 2c).
Thus, additional phantom studies evaluating the reproducibility of BMD measurements using this algorithm are necessary.
Test-retest reproducibility measurements using this instrument in our hands showed a coefficient of variation of 3.7%[14]].
However, careful estimation of the rotational correlation time is required to achieve precise and reliable distance measurements using this approach.
A scheme for calibrating and making measurements using this five-laser-beam probe is proposed and verified experimentally.
were satisfied, the measurements were regarded as stable, and the results obtained using a 2-s-long child time window were used.Figure 2 shows examples of accepted and rejected measurements using this procedure.
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Thioguanine, harmine, and phenylbiguanide were selected for IC50 measurement using this newly designed method with values of 4.5, 13.3, and 28.7 μM, respectively.
The intracellular fluorescence measurement using this probe mainly reflects the ability of stilbenoids to inhibit intracellular OH radical [37].
In all analyses we therefore used a laboratory determination whenever one was available and otherwise adjusted the field tester measurement using this correction factor.
The curve with heart activity (pulse) visible from the measurement using this method based on laser measurement principle can be seen in Figures 4 and 5.
To obtain the most accurate possible measurement using this platform, dPCR should be performed at 200 700 positive partitions per 765 chamber panel [ 31].
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