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Moreover, accurate GFR measurements using inulin or iothalamate infusions are undoable for large scale study populations.
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GFR and RBF were measured using inulin and PAH, respectively, according to established methods.
In the first two studies, GFR was measured using inulin clearance.
Glomerular filtration rate (GFR) was measured using inulin clearance and effective renal plasma flow (ERPF) using para-aminohippurate clearance.
To further monitor the integrity of the PGEC barrier, we also measured the paracellular passage of the extracellular marker inulin by determination of the permeability coefficient as evaluated by a diffusion assay using inulin as described previously [19].
The amount of fructose transferred and incorporated into inulin (FPOL) was determined from a reference plot relating inulin GPC elution area to inulin concentration, which was constructed according to the procedure described above using inulin precipitated with ethanol and subsequently freeze-dried.
The effect of hydralazine on glomerular filtration rate (GFR) in mice was also examined using inulin single injection plasma clearance.
Indeed, correct estimation of glomerular filtration rates implies its determination by using inulin or iohexol clearance, and radionucleotide.
Standards are used (inulin from dahlia tubers, Sigma-Aldrich; sucrose, Sigma-Aldrich) to confirm the identity of the sample peaks and to create standard curves to determine inulin and sucrose concentrations.
No comprehensive systematic review of the accuracy of glomerular filtration rate (GFR) measurement methods using renal inulin clearance as reference has been published.
For C-inulin measurements, samples were mixed with 5 mL of scintillation cocktail then dpm was measured using a Wallac 1414 Liquid Scintillation Counter.
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