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The use of a V-shaped alignment feature allowed for easy and reliable positioning of the tubing inside the detector, as demonstrated by a RSD of 1.9% (n = 10) in peak height when repositioning the tubing between measurements using flow injection analysis (FIA).
The impact of pemetrexed on cell killing was further analyzed by cleaved PARP measurements using western blot and Annexin V measurements using flow cytometry at 24-hours in cell treated with 0 100 nM pemetrexed.
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Cells were incubated in the dark, at 37°C for 30 minutes before immediate measurement using flow cytometry of gated monocytes and neutrophils.
This has recently been successfully combined with a fluorescent DNA dye to allow measurement using flow cytometry (17), but necessarily involves serial manipulation of the P. falciparum culture and exposing it to enzymes that are sometimes present in nonphysiological buffers.
The rarity of CEC and the controversy concerning the reliability of their measurement using flow cytometry have yielded conflicting as well as limited data in cancer pts (Strijbos et al, 2008).
Cell-size and cell-number measurements were conducted using flow cytometry.
Fluorescence measurements were made using flow cytometry throughout growth in wild-type, Δ sigB and Δ rsbV backgrounds.
The characteristics and reactivity of two anodes based on Pt supported on carbon nanotubes (CNTs) without or with defects induced by ball-milling are studied by SEM, TEM, cyclic voltammetry (CV) and single-cell measurements using a flow of pure H2 or containing 50 ppm CO.
We presented a study on coronary artery pressure measurements using blood flow simulation in realistic 3D reconstructed coronary arteries.
While cell size measurements using imaging flow cytometry show that the cell volume of the WT tetraploid is roughly 60% larger than that of the WT diploid, this corresponds to a cell surface area increase of only 36%.
In order to guarantee that the optics, laser, fluidics and fluorescence intensity were stable during all measurements calibration was performed using Flow Check Pro Fluorospheres (Beckman Coulter) and Cyto-Cal Multifluor + Violet Fluorescence Alignment Beads (Thermo Scientific, Fremont, CA, USA).
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