Peripheral quantitative computerized tomography (pQCT) was performed on formalin fixed left tibia for bone mineral density measurement using a method well established previously in our lab [32] using a STRATEC XCT 960 M unit (XCT Research SA, Norland Medical Systems, Fort Atkins, WI) specifically configured for small bone specimens.
Kinetic and transport parameters are estimated by minimizing the sum-of-squares error between the model and experimental measurements using a simplex method.
Meanwhile, a new topographic field is established around LMS by merging Global Topography V18.1 (Smith and Sandwell 1997) data with GPS measurements using a similar method.
Simulation was also tested by experimental measurements using a foil activation method.
An unknown transient heat source in a three-dimensional participating medium is reconstructed from temperature measurements using a Bayesian inference method.
Most of the unknown kinetic and thermodynamic parameters of the model were estimated from experimental measurements using a stochastic optimization method based on a genetic algorithm.
Experimental measurements using a static headspace method were carried out for pure water (Hvoc,water), for pure silicone oil (Hvoc,solvent) and for mixtures of varying composition (Hvoc,mixture).
And then, the amount of DOX in micelles were analyzed with fluorescence measurements using a standard curve method on a Photon Technology International PTII) Fluorescence Master System with software Felix 4.1.0 (λex = 480 nm; PTI, Inc., Lawrenceville, NJ, USA).
C) To experimentally address the referees' concern we have now performed additional stability measurements, using a more sensitive method, single-molecule FRET analysis.
Since IOP was a main study endpoint, particular care was taken to obtain unbiased measurements using a 2-person method in which one adjusts the dial in a masked fashion and the second reads and records the value.
