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PI at a final concentration of 10 μg/ml was added to each sample immediately before the measurements to exclude non-viable cells from the analysis.
We also conducted several control measurements to exclude the possibility that LMAN lesions by themselves trigger reactive changes in HVC circuitry or to the bird's song that might occlude or otherwise alter spine dynamics in HVC.
Finally, we purposely avoided the use of objective measurements to exclude patients with possible CLI from the study and instead included all patients with documented limb ischemia with rest pain or tissue loss of sufficient severity to warrant urgent hospital admission.
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The samples were gently ground prior to measurement to exclude the influence of particle size and surface area.
A wrist cuff was inflated to suprasystolic pressures 1 min before each measurement to exclude the hand circulation.
Patient underwent full blood count and haemoglobin electrophoresis to exclude thrombocytosis and sickle cell anaemia, and serum angiotensin-converting enzyme (SACE) measurement to exclude sarcoidosis.
Before each experiment, the urinary bladder was emptied, and total urine production during each experiment was collected for measurement to exclude renal glucose excretion.
This was probably because participants were provided with a light LF meal on the day that preceded measurement to exclude the acute effects of variations in fat intake.
The patients changed into a hospital gown before WBC measurement to exclude any radiocontamination that may have been present on their clothing.
The Xcalibur instrument software uses exclusion criteria in a nano-LC MS measurement to exclude selected MS1 parent masses for MS2 fragmentation in order to obtain as many protein identifications as possible (www.thermoscientific.com).
To provide a high level of reproducibility of clinical measurements and to exclude a already known influencing factor, only single-rooted teeth were considered in this study.
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