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They found that the changes in the fluorescence intensity resulting from different levels of extracellular ammonium, as measured by the AmTrac sensor, correlated with levels of ammonium transport determined from measurements of substrate currents.
The FE model is validated against in situ experimental measurements of substrate distortion.
Direct measurements of substrate binding will be required to determine which is the case.
Besides the small number of patients included, a major limitation of this study is the substantial variability of the measurements of substrate metabolism themselves.
Spectrophotometric measurements of substrate oxidation by nLac and rLac were carried out in a 2 ml reaction volume containing the test substrate in 50 mM sodium citrate buffer (pH 4).
Indeed, cyclic voltammetry measurements of substrate 3r indicated that it possessed a half wave oxidation potential of +1.53 V vs. SCE, whereas the corresponding amide was greater than +2.5 V vs. SCE.
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The aim of this study was to compare myocardial metabolic changes by direct measurements of substrates, such as glucose, lactate and pyruvate, using microdialysis (MD) in in-vivo porcine endotoxemic and hemorrhagic shock.
Kayalar, C., Rosing, J. A. N. & Boyer, P. D. An alternating site sequence for oxidative phosphorylation suggested by measurement of substrate binding patterns and exchange reaction inhibitions.
Lance assay for measurement of substrate phosphorylation and analysis of kinetic parameters.
The traditional experimental practice in enzyme kinetics involves the measurement of substrate or product concentrations as a function of time.
Measurement of substrate curvature, in situ and ex situ, yields thermal stress.
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