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Short-term measurements of integrated net water flow did not detect variation among treatments.
The device is characterized at room temperature through the measurements of integrated electroluminescence (EL), light intensity-voltage characteristics, spectral photoluminescence (PL), EL, and electro-photoluminescence (EPL) techniques.
Total Li+ content per unit cell and the cation distribution over a spinel lattice in LixMn3−xO4 have been studied by measurements of integrated intensities of X-ray reflections, and by structure refinement using Rietveld profile analysis.
Dedicated machine vision software calculated the size, in kilobase pairs (kb), of each fragment based on measurements of integrated fluorescent intensity, resulting in the high throughput, massively parallel generation of ordered restriction maps, or 'Rmaps', from individual genomic DNA molecules [ 66].
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DOI: http://dx.doi.org/10.7554/eLife.02137.010 Although the method we employed to measure centromeric ratios is internally controlled, it relies on measurement of integrated fluorescence of whole cells, including highly dilute cytoplasmic CENP-A.
A flow plethysmograph measures the volume displacement of the chest movement simultaneously with the spirometric measurement of integrated flow at the mouth.
PCR conditions consisted of one cycle of denaturation (94° for 2 min), 39 cycles of amplification (94° for 15 sec, 50° for 30 sec, 68° for 8 min), and one cycle of 68° for 7 min. 5 µl of resulting PCR product was used as the template for measurement of integrated HIV-1 DNA in a nested real-time quantitative PCR.
The fluorescence 2-area (FL2-A), a measurement of integrated fluorescence signal, was used as the parameter linearly correlated to DNA content (Dart et al. 2004).
These issues are largely obviated in optical mapping since the mapping substrate is just naked, fully deproteinized DNA molecules, and "distances" are robustly estimated by measurement of integrated fluorescence intensity [ 53, 63] using a dye (YOYO-1), whose measured fluorescence intensity is somewhat insensitive to base composition or extent of DNA elongation.
As an added bonus, the recovered atomic oxygen density profiles can be used to reconstruct the vertical profiles of the specific nightglow volume emissions, thus producing vertical profiles from measurements of their integrated intensities.
The expression level of BiP mRNA in the SON was quantified by measurements of the integrated optimal densities (optic densities × area) of the film images using ImageJ software (version 1.44k; http://rsbweb.nih.gov/ij/).
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