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After two sorting rounds of recombinant virus and measurements of expression efficiency for more than 20 viral clones, the over-expressed recombinant virus Bm-P12A3C was obtained.
As measurements of expression levels, we used two sets of mRNA abundance data measured by high-density oligonucleotide arrays [ 20] and cDNA microarrays [ 21].
Second, measurements of expression levels in the laboratory can be used to filter data sets of dispensability estimates, removing variates that are unlikely to reflect real biological effects.
CD73 is tightly regulated in response to lung injury in a variety of lung diseases, and measurements of expression have proven to be a useful approach in understanding the regulation of adenosine synthesis and signaling during disease processes (9, 24).
To demonstrate the applicability of bgaB as a reporter for quantitative measurements of expression levels, a promoter library was constructed using a method described by Jensen and Hammer (1998a, b).
This means that for each gene represented on the array, there are between 160 and 240 measurements of expression level.
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Wilhelm, B. T. & Landry, J. R. RNA-Seq-quantitative measurement of expression through massively parallel RNA-sequencing.
The experimental pipeline allowed construction and measurement of expression levels of the library in just 4 days.
Second, we have ignored error in the measurement of expression levels.
Noise was added both in the dynamics of the networks and on the measurement of expression data.
The entire procedure from RNA isolation to the measurement of expression profiles is outlined in Figure 1.
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