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The workflow has three main components: a semi-automated image processing pipeline to track and record insect trajectories, a trajectory visualization tool for qualitative analysis of insect movements and the quantitative trajectory measurements for statistical hypothesis testing.
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The final agreement was calculated and used as final measurement for statistical analysis.
The performance assessed by root mean square error (RMSE), a widely used measurement for statistical regression analysis, are reported in Table 4. From Table 4 we can see that the proposed method always has better memory prediction performance.
We would like to thank Kim Piera and Barbara MacHunter for technical assistance; Jane Thomas, Mark McMillan, Karl Blenk, Antony Van Asche, Steven Tong and Paulene Kittler for RH-PAT measurements; Ric Price and Joseph McDonnell for statistical advice; and the medical and nursing staff of the Royal Darwin Hospital Intensive Care and Hospital in the Home units.
Quantitative colocalization measurements used for statistical analysis were performed on each pixel in the area of interest.
Indeed, because of our 'systems level' approach it is likely that the neural response strengths used as the measurement variable for statistical analyses is a reflection of multiple different neural processes sensitive to stimulus history.
Measurements at baseline and 6 months were duplicated (with repositioning at each measurement), and the average of both measurements was used for statistical analyses.
When the total color difference (ΔE) of two measurements did not exceed the threshold of 1 ΔE unit, the mean value of these measurements was used for statistical analysis.
Average of two measurements per eye was considered for the analysis, and if two measurements differed more than 2 mm Hg, the third one was obtained and average of closest measurements was considered for statistical analysis.
Sitting blood pressure was measured three times with use of a mercury sphygmomanometer after the subjects had a 5 min rest, and the average of the three measurements was used for statistical analysis.
The average of duplicate measurements was used for statistical analysis.
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