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The COUP-TF1 gene (Nr2f1) was upregulated in transgenic and tumor in comparison to the normal tissue according to gene expression measurements (fold changes 6.26 and 2.62 in transgenic and tumor, respectively).
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This is a result of the fact that all variances of individual gene levels are aggregated into a single decision: differentiated gene or a single measurement: fold change or p-value.
In addition to the investigation of the actual expression intensities, fold changes derived from resulting gene expression intensities were compared to fold changes based on quantitative measurements of RNA abundance as determined by qRT-PCR.
Values correspond to fold changes.
Each probe has a score on the basis of its fold change relative to the standard deviation of repeated measurements for this probe.
Fold change (qRT-PCR) Fold change log2 FC) FDR Sig.
The measurement of fold changes of genes between HBRR and UHRR was highly correlated with data obtained from qPCR.
In order to confirm the microarray results and to obtain an independent measurement of fold changes between mutants and controls, we used QRT PCR.
We compared miRNA quantification by the ddPCR system and quantitative real-time PCR (qPCR) to demonstrate that the ddPCR system was superior to qPCR both in its detection limit and smaller fold changes measurement.
The measurements are presented as mean fold changes ± standard error of the mean (SEM).
Both Strength and GPI quantify log2 differential values (i.e., log2 fold changes) of measurements in the HYP.
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