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A1C was also measured over time as an updated mean of annual measurements, calculated for each individual from baseline to each year of follow-up, with the last observation carried forward for missing data.
Proliferation was measured every 4 hours for 84 hours, with respective AUCs for serial measurements calculated for each condition for red (proliferating) or dual-labelled (apoptotic) cells.
The updated mean values of HbA1c, LDL cholesterol, HDL cholesterol, triglycerides, BMI, blood pressure, and eGFR over time were measured first at baseline and second as an updated mean of annual measurements, calculated for each participant from baseline to each year of follow-up.
This enables, in the case of the occurrence method, the minimization of the extreme values effect and means that only the relevant measurements calculated for the selected individuals are retained.
From the distribution of measurements calculated for body mass and fitness levels, the BMI and CRF values were divided into low, middle, and high groupings [ 32, 33].
In particular, the possibility to use different scales, from higher (0.5 8.5 ppm) to lower (0.5 4.7 ppm) amplitude values, allowed to better adjust the base line, taking into consideration peak amplitude measurements calculated for water and other metabolites in the automatic and manual determination of spectral phase and frequency.
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The updated mean values of HbA1c, LDL cholesterol, BMI, blood pressure, and eGFR over time were measured first at baseline and second as an updated mean of annual measurement, calculated for each participant from baseline to each year of follow-up.
The purity of the resulting fluorescein-labeled cyclic peptide was assessed by analytical RP-HPLC (>99%) and its identity was confirmed by exact mass measurement (calculated for C58H69N8O14S+ = 1133.4648; observed = 1133.4645).
The adjusted within-person correlation for daily measurements, calculated directly from the Tobit model in Table 3, was 0.47.
After excluding extreme outlying measurements for individuals based on their personal range of blood pressure measurements, median systolic and diastolic blood pressure measurements were calculated for each individual using all results available within +/− 1 year of the dietary questionnaire (median 6 measures per individual, range 1 207).
Blood pressure was measured twice in the sitting position with a 10-min rest before the first measurement, and the mean values of these two measurements were calculated for both systolic blood pressure (SBP) and diastolic blood pressure (DBP).
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