Fluorescence measurements are fit starting at 5 μs.
Optical density was measured on Thermo Biomate 3S at 600 nm for triplicate cultures, and the averaged measurements were fitted to Richard's curve (Richards 1959) by the least squares method in Scipy package.
First, the measurements were fitted for b-values higher than 250 s/mm2 to estimate the parameter d using a mono-exponential term.
The results obtained from these measurements were fitted using the modified Apelblat equation, the combined nearly ideal binary solvent/Redlich Kister (CNIBS/R K) equation and the Jouyban Acree equation, respectively.
The measurements were fitted with a double exponential function from which the activity per unit mass (Bq/g) of free 213Bi (obtained as the time-zero intercept) and 225Ac-anti-PD-L1-BC 225Ac-anti-PD-L1-BC 225Ac-anti-PD-L1-BC 225Ac-anti-PD-L1-BC 225Ac-anti-PD-L1-BCsponds to the 10-day half-life of 225Ac) could be determined.
The titration data from fluorescence measurements were fitted with either a Hill equation or a model with one or two binding sites (n = 1 or 2) as in Eq. 2. Θ = F − F min F max − F min = ∑ i = 1 n ( [ P ] t + [ L ] + 1 / K a i ) − ( [ P ] t + ( L ) + 1 / K a i ) 2 − 4 [ P ] t [ L ] 2 [ P ] t (2).
Absorbance measurements were fit to a linear correlation model (R2 = 0.99).
The averaged data from eight independent measurements were fitted to the Hill equation using Origin7 software (OriginLab).
The data from equilibrium fluorescence and stopped-flow measurements were fitted globally to a sequential mechanism with an on-pathway intermediate according to equation 1 using pro Fit 6.1.11.
Since individual measurements of nematodes could not be distinguished from those of extraneous matter, all aspirated measurements were fit to a weighted sum of the lognormal distribution and the predicted nematode frequencies at each time point.
