Also, the significant socioeconomic gradient in PF at the second measurement was maintained.
Body temperature (as assessed via rectal measurement) was maintained at 37°C with a heating pad (Panlab S.L., Barocelona, Spain).
Measurements are maintained in the switches and are asynchronously sent to a SDN controller.
The temperature during the measurements was maintained at approximately 302 K (84°F), leading to the -176.8 dBm/Hz expected value for the two-sided PSD of the thermal noise.
The flow rate of HBS-EP buffer during all measurements was maintained at 10 μl/min.
The sample temperature for all CD measurements was maintained at 293 K. Sedimentation velocity analyses were conducted using a Beckman Optima XL-I analytical ultracentrifuge in the University of Massachusetts Medical School Ultracentrifuge Facility.
In order to establish an assay which would allow the effective measurement of fluorescence of epithelial and mesenchymal markers, we first determined the confocal settings in the range of which linearity of fluorescent measurements is maintained.
In addition the original measurement values were maintained in order to provide input for the realizations with treatment bias.
For all three measurements, contractions were maintained for 4 seconds, with a rest of 30 seconds between each measure.
Throughout AFM indentation measurements, cells were maintained at 37°C in a live imaging buffer containing 10 mM HEPES, 150 mM NaCl, 3 mM KCl, 22 mM sucrose, 10 mM glucose, 1 mM MgCl2, and 2 mM CaCl2 at pH 7.4 (31).
