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Because PMTs exhibit good SNR and repeatable measurements over more than their range of linear response to measured signal strengths, measurement standard deviation is not an appropriate indicator of linearity, and the PMT's must be characterized prior to calibration.
The optimization procedure estimates the parameters in equations (2) to (9) based on the optimization criterion (10) where the deviation of calculated and measured concentrations divided by the measurement standard deviation s j, squared and summed over all metabolites J and all time points N, has to reach a minimum.
The measurement standard deviation is 0.5 L/s for a pump with the design flow rate of 37.9 L/s.
Again measurement standard deviation is normalized to average unit thickness and displayed as percentage error (Figure 7).
A single shot resolution of the implemented converter is 63.3 ps and the measurement standard deviation is about 61.7 ps within the measurement range of 5 ns.
B. Filled symbols represent uncertainties calculated as the percentage difference between measurement standard deviation and the average thickness for each unit at each location.
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Average differences between measured and predicted yields were generally within the range of one to two measurement standard deviations.
The lower Protein A ligand density on MabSelect SuRe caused larger measurement standard deviations.
Owing to the ease of the yEGFP measurement, standard deviations are generally less than 3%.
In this way, measurement standard deviations for each compound could be estimated independently of other compound concentrations.
The device used was Tinius Olsen, tension of samples 3 N, average of 10 measurements, standard deviation 35±5%5%.
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