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Image analyses were performed using the Volocity Measurement Software (Improvision).
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Cilia labelled with anti-acetylated α-tubulin antibody were imaged at the confocal microscope using a 40× oil immersion objective and measurements were performed with Volocity software (Improvision) on the maximum projection obtained from the z-scans.
Colocalisation analysis, Pearson's correlation, line scan analysis and fluorescence intensity measurements were carried out using Volocity software (Improvision, Perkin Elmer, Waltham, MA, USA).
The cell shape and area of MDA-MB231 cells were measured and calculated with OpenLab software (Improvision).
Measurements were acquired using Velocity 3D imaging software (Improvision PerkinElmer Company).
These regions were measured in 3D by using Volocity software (Improvision).
Optical sections of 0.4 μm were acquired and processed with Volocity software (Improvision, Perkin Elmer) for image rendering and measurements.
Images were captured and analyzed using Openlab software (Improvision).
Image analysis was performed using Openlab 4.0.4 software (Improvision).
Images were acquired and digitally merged with Volocity software (Improvision).
OpenLab software (Improvision, Inc., Portage, MI) was used for image acquisition and hardware control.
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