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Calculations are done based on a statistical analysis comprising of difference between average data in adjacent well zones, and the difference which is expected from measurement of variance values in zones.
The measurement of variance in the distribution of gene expression levels was not normally distributed within the group of control children.
Interestingly, as was suggested by the measurement of variance in gene expression levels in high and low freezing mice, we only saw significant changes in the separation of log-normal gene expression distributions in the hippocampus and not in the amygdala.
We assessed collinearity between variables through measurement of variance inflation factor (VIF).
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Importantly, we have found that measurements of variance in log-transformed levels of gene expression are sensitive to both biological and technical factors (see below).
As with all measurements of variance, these accuracy estimates should be considered as lower bounds due to experimental noise.
When analyzing the primary outcome measure, Roland, the primary analysis will be a repeated measurement analysis of variance, hence estimating the original measurements.
Measurements across multiple timepoints were compared using a repeat measurement analysis of variance, while single time point measures were compared with a two-sided, independent-samples Student t test with adjustment for birth-weight z score and participant sex.
Results of repeated measurements were compared using a repeated measurement analysis of variance (One-Way ANOVA).
A standardized measurement of the variance keeps the variances within the same order of magnitude whatever the means.
Repeated measurements will be analysed using a repeated measurement analysis of variance.
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