Sentence examples for measurement of transcripts from inspiring English sources
Measurement of transcripts requires that RNA samples be isolated from tissue, for which the collection rates and cytosol volumes are difficult to estimate.
This technology therefore in principle allows the measurement of transcripts in an orientation-specific manner.
For example, the measurement of cytokine proteins in serum is interspersed with measurement of transcripts, and it is not always evident in the text what is being measured.
DGE profiling provides a precise measurement of transcripts levels in different samples and allows the intercomparison among samples to identify DEGs.
Furthermore, RNA-seq provides a far more precise measurement of transcripts and has been successfully used for transcript profiling in many plant species [ 33, 34].
Primers for premature miR156b rather than mature miR156 sequence were used so that measurement of transcripts originating from other miR156 genes would be excluded.
Primers used for measurement of transcript levels were as follows.
mRNA sequencing with shotgun, massively parallel sequencing platforms has recently shown utility for measurement of transcript abundance, splice isoforms and allelic influence on gene expression [31] [50].
Thus, we were able to derive at least one measurement of transcript length for nearly half of the 25,000 genes the human genome is predicted to encode, and from 6,238 of those genes at high (90%) confidence.
Characterization of RNA transcripts by length does not have the resolution to identify precise sites of transcript initiation or termination, precise splice sites, or even exon-intron structure, but it does provide an unbiased measurement of transcript length, a quantity that is relatively difficult to obtain through full-length cDNA sequencing alone.
A more precise measurement of transcript abundance of these genes was performed by qRT-PCR, which revealed that differential expression was significantly greater than had been detected by microarray: the range of fold difference between ALL and AML by gene expression array was 1.0 2.8 and by qRT-PCR 2.3 45.6 (Figure 3B).
