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Lance assay for measurement of substrate phosphorylation and analysis of kinetic parameters.
Measurement of substrate curvature, in situ and ex situ, yields thermal stress.
The traditional experimental practice in enzyme kinetics involves the measurement of substrate or product concentrations as a function of time.
This paper aims at the estimation of biomass concentration of fed-batch fermentation processes by simultaneous measurement of substrate consumption, oxygen uptake and carbon dioxide production.
An example is the flux balance approach (FBA) of Palsson and coworkers, which has had considerable impact in this regard as it combines the simple measurement of substrate uptake with the postulated regulatory choice of maximizing the yield of biomass.
Thus, FRET efficiency represents a linear measurement of substrate cleavage that is instrument-independent (Fig. 1Aii and B).
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As suggested above, a potential limitation of measurement of substrate-induced currents by the TEVC technique is that electroneutral movement of compounds through the carrier cannot be detected.
We suggest the measurement of substrate-specific anti-factor Xa activity in patients treated or suspected of being treated with oral anti-factor Xa agents such as rivaroxaban, apixaban or endoxaban.
PAT2 function was characterised by: radiotracer uptake and competition (cis-inhibition) studies; radiotracer efflux and trans-stimulation; and measurement of substrate-induced positive inward current by two-electrode voltage-clamp.
Placing the liver on a balance pan not only allows for monitoring liver mass, whose baseline constancy is one surrogate marker for excellent perfusion quality, but also allows the measurement of substrate- and hormone-induced changes in the hepatocellular water content in the absence of alterations of perfusion pressure and flow (vom Dahl et al. 1991).
They found that the changes in the fluorescence intensity resulting from different levels of extracellular ammonium, as measured by the AmTrac sensor, correlated with levels of ammonium transport determined from measurements of substrate currents.
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