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Although the CV is the standard measurement of expression variability in the literature, we also employed an alternative measure which has recently been proposed by Alemu et al. [ 30].
Wilhelm, B. T. & Landry, J. R. RNA-Seq-quantitative measurement of expression through massively parallel RNA-sequencing.
We found that the increased constituent ratio of original follicle and decreased diameter of all levels of follicular oocytes(compared with control, with statistically significant differences, P < 0.01).After the measurement of expression of SCF/c-kit by qRT-PCR and Western Blotting, the mRNA and protein expression of SCF/c-kit in ovarian were both decreased.
The experimental pipeline allowed construction and measurement of expression levels of the library in just 4 days.
Second, we have ignored error in the measurement of expression levels.
The entire procedure from RNA isolation to the measurement of expression profiles is outlined in Figure 1.
Noise was added both in the dynamics of the networks and on the measurement of expression data.
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After two sorting rounds of recombinant virus and measurements of expression efficiency for more than 20 viral clones, the over-expressed recombinant virus Bm-P12A3C was obtained.
As measurements of expression levels, we used two sets of mRNA abundance data measured by high-density oligonucleotide arrays [ 20] and cDNA microarrays [ 21].
Second, measurements of expression levels in the laboratory can be used to filter data sets of dispensability estimates, removing variates that are unlikely to reflect real biological effects.
CD73 is tightly regulated in response to lung injury in a variety of lung diseases, and measurements of expression have proven to be a useful approach in understanding the regulation of adenosine synthesis and signaling during disease processes (9, 24).
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measurement of microcystin
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