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The enhanced media feed was most beneficial when applied one day before the intended measurement day, not two days before (strategy 1) nor over two consecutive days (strategy 2).
To calculate the average maximum bending moment for each measurement day, the 10 highest measured values were used.
On each measurement day, the subjects were measured on three different conditions: resting condition (RC), sling condition (SC), and abdominal press condition (APC).
The same procedure was repeated for each subject, on each condition, and on each measurement day, with the values of the three measurement days being averaged (cf. [ 14]).
Obtained data from both platforms were normalized according to sample weight and to the measurement day to minimize process error over the course of many days of measurement.
Stress reduced PPI of Long Evans females on every measurement day but not of other groups.
Data were normalized regarding sample weight, measurement day and median of the respective metabolite per analysed batch.
In the early morning of the measurement day, the twigs kept in the polythene bags were cut off and quickly transported to laboratory.
At the time of analysis, one patient was excluded because she developed meningitis with severe neurologic impairment on the measurement day.
Stress did not affect 15-min activity levels of Long Evans females but decreased 15-min activity levels of Sprague Dawley females on every measurement day.
A batch of 100 μL MTT solution with 900 μL medium was incubated with the cells in the wells at 37 °C for 3 h (n = 3 for each measurement day).
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