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qPCR was performed in 25 μl reactions using LightCycler 480 SYBR Green I Master reaction mix (Roche, Indianapolis, IN, USA) and a LightCycler 480 instrument (Roche) A Basic Relative Quantification workflow was used that included fluorescence measurement at each PCR extension step and a final melting step measuring fluorescence from 95-55°C 95-55°Ct Curve Genotyping.
Weight was measured without shoes, excess clothing and items in pockets by a single measurement at each survey.
To map the sample topography and potential quasi-simultaneously, a software feedback loop alternatingly performs first topography and then potential measurement at each scan pixel.
The target measurement at each scan k is {boldsymbol{z}}_{k} ={{{boldsymbol{H}}}}boldsymbol{x}_{k} + boldsymbol{w}_{k} (7).
The dark reference image was first subtracted from both the white reference and the actual measurement at each wavelength in order to correct for thermal dark current [6].
Stresses were measured with a polariscope (Vishay LF/Z-2, Malvern, PA, USA), and photographs were taken from each measurement at each point (Figure 1).
Similar(18)
One height and two width measurements at each site were measured.
For each of the supraspinatus muscles, the scanning session consisted of measurements at each of the three measuring sites, longitudinally as well as transversely [ 19].
Our measured data are averaged values from three measurements at each radial distance.
The thickness at the medial and lateral condyles was measured by taking the mean of three measurements at each site.
Two measurements at each temperature were recorded, and data collected every 0.5 °C.
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