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Following the mechanosensitivity measurement, animals received acupuncture treatment, in which an acupuncture needle was applied to ST36 acupuncture point of the ipsilateral leg for 20 min with gentle manipulation, and further measurements were taken to evaluate the effects of the treatment.
To counteract cholinergic bronchoconstriction at the end of each measurement animals were treated with the bronchodilator salbutamol.
Another important feature of the assays is that paralysis is a binary measurement – animals are either paralyzed or they are not.
Following baseline arterial pressure and ICP measurement, animals remained on the same base plate and stereotaxic frame, which was positioned on the CT scanner table.
For body weight measurement, animals were housed at a maximum density of five per cage and weight was recorded up to 52 weeks of age.
For food intake measurement, animals were housed at a density of four per cage and food weighed before and after each 72-h period for 10 weeks.
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To enable international comparison of veterinary antimicrobial consumption data, harmonized units of measurement, animal weights and animal (sub) categories are needed.
Using these measurements, animals were divided into groups displaying approximately equal levels of FPS prior to drug treatment and extinction training.
After baseline measurements, animals were bled until the stroke volume index (SVI) decreased by 50% (T 0).
After such baseline measurements, animals received a rapid intravenous infusion (over 30 min) of 1 L of trial solution.
After baseline measurements, animals were randomly allocated to receive only NS or RL titrated to maintain pulmonary artery occlusion pressure at baseline level.
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