This shows that the stochasticity of gene expression, even measured with steady-state metrics, is controlled by - and hence can reveal - the dynamics of the promoter.
The next day after FMISO PET, the tumoral blood flow (TBF), tumoral metabolic rate of oxygen (TMRO2), oxygen extraction fraction (OEF), and tumoral blood volume (TBV) were measured with a steady-state inhalation method under artificial ventilation of 15O CO2, 15O O2, and 15O CO gases mixed with room air and oxygen [10, 11].
Stomatal conductance was measured with a steady-state porometer (SC-1, Decagon Devices, Pullman, WA, USA) on the first true leaf in each plant, a completely expanded mature one.
Luciferase expression was measured with the Steady-Glo Luciferase Assay System (Promega) directly from an aliquot of cell culture: 10 μl of culture (in EMM) were mixed with 15 μl of Passive Lysis Buffer (Promega) and 25 μl of Steady-Glo substrate solution (Promega) in a 96-well microtiter plate; luminescence was measured with a plate reader (Tecan).
Step-up and step-down responses of several typical fluid-filled bushing configurations are measured along with steady harmonic time histories of transmitted force and internal pressures.
Emax (15.5 ± 0.6 mN, Figure 1F) and EC50 (21.8 ± 1.2 mM K+) were not significantly different from the steady state values measured with repetitive depolarization (vide supra).
Blood pressure in restrained conscious mice at steady state was measured with a programmable tail-cuff sphygmomanometer (MK-2000A; Muromachi, Tokyo, Japan).
The UPQC can easily compensate for voltage flicker using only the main controller in Section 3. Figure 12 shows the steady-state response measured with the osciloscope when the UPQC is compensating for voltage flicker.
Current/voltage relationships measured with ramp protocols matched current/voltage relationships determined from steady state currents evoked by depolarizing steps (100 ms, 10 mV increments; Fig. S1).
The steady state fluorescence anisotropy was measured with excitation at 340 nm and emission between 540 and 560 nm.
Steady-state oxygen evolution rates were measured with Clark type oxygen electrode (Hansatech DW1, UK) at saturating light intensity.
