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Serum levels of estradiol (E2), estrone (E1), testosterone (T), and androstenedione (adione) were measured with previously described radioimmunoassay methods [ 13].
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Frequency of participation in cognitive activities was measured with a previously established scale [ 27].
OHL was measured with the previously validated word recognition test (REALD-30) [ 23].
Serum PRL concentrations were measured with a previously validated heterologous radioimmunoassay (RIA) [ 51].
Serum P4 concentrations were measured with a previously validated H-RIA [ 51, 54].
Body weight was measured with a previously calibrated electronic scale (model BC-533; Tanita, Tokyo, Japan), with participants wearing minimal clothing.
Serum antibodies against ER were measured with a previously described indirect enzyme-linked immunosorbent assay (ELISA) [ 29, 30].
This was measured with a previously validated teaching self-efficacy instrument for STEM GTAs (DeChenne et al., 2012b).
C-reactive protein was measured with a previously validated canine-specific immunoturbidimetric assaye on a fully automated chemistry/immunoassay analyzerf [ 16].
Opportunities for career development were measured with items previously tested in studies on Slovenian health-care management [ 16, 17, 21] and the theoretical background in this area [ 5, 18].
Concentrations of afamin were measured with a previously described double-antibody sandwich ELISA test, using a biotinylated affinity-purified polyclonal antibody for binding to streptavidin-coated microtiter plates and the peroxidase-conjugated monoclonal antibody N13 for detection.
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