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Supernatants were centrifuged and IFN-α and IFN-γ levels measured with human interferon-α multi-subtype ELISA (PBL Interferon Source, USA) and IFN-γ ELISA (R&D Systems, UK) kits.
Soluble APRIL concentrations were measured with Human APRIL (Platinum ELISA, eBioscience, Austria).
RANTES, MCP-1, and IL-6 were measured with human two-site ELISAs (R & D Systems, Abingdon, UK).
ApoB-100 was measured with Human apolipoprotein B ELISA kit (Mabtech) according to instructions provided by the manufacturer.
IL-12, TNF- α and IL-1 β levels of plasma and supernatants were measured with human ELISA kits (BD Biosciences), according to the manufacturer's instructions.
IL-6, TGF-β1 and IL-17 levels of plasma and supernatants were measured with human ELISA kits (R&D Systems, Inc., Minneapolis, MN, USA), according to manufacturer's instructions.
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Cushion transmissibilities were also measured with nine human subjects, having an average seated weight similar to the dummy.
Three biological replicates from each cell type were measured with PrimeView Human Gene Expression Arrays, covering more than 36,000 transcripts and variants.
To evaluate transgene expression from mc-oriP-IFNγ or mc-CMV-IFNγ in EBV-negative and -positive cells, the concentration of IFNγ in the culture supernatant of transfected cell lines was measured with a human IFNγ ELISA kit (R&D Systems, Minneapolis, MN) according to the manufacturer's protocol.
IL-6 was measured with a human IL-6 ELISA kit from RayBiotech (Norcross, GA).
Kit (eBioscience) and IL-18 was measured with a Human IL-18 ELISA kit (MBL).
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