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Specific IgG titers in whey and serum were measured by indirect ELISA, and total IgG concentrations were measured using sandwich ELISA.
TNF-α and IFN-γ concentrations in peritoneal exudates were measured using sandwich ELISA per the manufacturer's instructions (R&D Systems).
Concentrations of CXCL8/IL8 in cell-free supernatants were measured using sandwich ELISA kits (R&D Systems) and calculated using 4-parameter-log-fit curves according to manufacturer's protocols.
The levels of BDNF in brain tissues were measured using sandwich ELISA 7 days after MCAO.
BMP-7 levels in cartilage tissue were measured using sandwich enzyme-linked immunosorbent assay.
Complement activation product C3a in plasma was measured using sandwich ELISA kits (Quidel, San Diego, CA).
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BDNF protein concentrations were measured using sandwich-style ELISA BDNF Emax Immuno-Assayy System kit; Promega Corporation, Madison, WI) according to the manufacturer's instructions (See Supplementary information).
The through-thickness and in-plane capacitances are measured using sandwiching and coplanar electrodes, respectively.
IgM, IgG and IgA concentrations were measured using sandwich-type ELISA assays prepared in our laboratory.
SP-A and SP-D levels were measured using sandwich-type enzyme immunoassay kits (SP-A test-F, Kokusai Shiyaku Co., Hyogo, Japan; and SP-D kit, Yamasa, Yamasa Shoyu Co., Tokyo, Japan).
Serum GIP concentrations in samples of venous blood obtained after an overnight fast were measured using a sandwich enzyme-linked immunosorbent assay kit.
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