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This possibility was eliminated by control experiments in which proton translocation was measured using regular NADH, which is used by the alternative NADH dehydrogenase (ND-2).
For culture medium, the medium was removed from the culture dish immediately after the dishes leaving the incubator, and the pH was measured using regular lab oratory pH meter.
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Additionally, surface meteorological parameters, temperature (T), ambient pressure (P), relative humidity (RH), wind direction (WD), wind speed (WS), and solar radiation (SR) were also measured using a regular meteorological station.
The weight and height of each participant were measured using a regular scale, and the body mass index (BMI) was calculated as the ratio between weight and squared height.
To reduce variability, BMD will be measured using a standard protocol (including regular use of a phantom spine).
Blood pressure was measured using a calibrated mercury sphygmomanometer, regular cuff size (unless obese) following a 5 min seated rest period.
The number of regular patterns was measured using a threshold value for CV2 ranging from 0 to 2 with an increment of 0.02.
The strength of synchronization of regular patterns was measured using a standard score, the Z score of the amplitude of the central peak of the cross-correlogram [21]: Z = (Nc−Ne)/ SDe), with Nc the number of spikes in the central peak (bin = 5 ms), Ne the mean number of spikes in a 2 sec window between −1 and 1 sec, and SDe its standard deviation.
Because the active ingredient in VIAject is recombinant human regular insulin, human regular insulin and VIAject insulin were measured using the same insulin assay, whereas insulin lispro was measured with a lispro-specific assay, minimizing detection of endogenous insulin.
Vertical motions and bending moments were measured using potentiometers and strain gages, respectively in regular waves.
At regular intervals of time, rheological behavior at different shear rates was measured using Physica Rheolab MC1.
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