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Absorbance is measured using microplate reader (Tecan, Zanker Road, San Jose, CA) at 570 nm, with reference values at 600 nm.
MMP2 activity was measured using microplate reader (BioTek Instruments, Winooski, VT, USA) at 450 nm, subtracted by readings at 540 nm.
The absorbency of each well (OD490 nm value) was measured using microplate reader (Bio-Rad).
Initial cell density and bacterial growth every after 24 h were measured using Microplate Reader (Poweam WHY101) at 590 nm.
The fluorescence intensity and absorbance of the samples were measured using microplate reader Infinite M200 (Tecan Ltd., Switzerland).
The absorbance was measured using microplate reader at 340 nm after every 12 s for 120 s.
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DNA-binding activity of NF-κB p65 was then measured using microplate-based ELISA according to the manufacturer's protocol (Active Motif).
Total serum IgE levels were measured using microplates coated with anti-mouse IgE R35-722; BD Biosciences).
Absorbance at 540 nm was measured using a microplate reader (iMark microplate reader; Bio-Rad).
Relative light units were then measured using a microplate luminometer (BioTek, Synergy 2 luminescence microplate reader).
The absorbance value at a wavelength of 570 nm was measured using a microplate reader (Thermo Fisher Scientific, Grand Island, NY, US)).
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