Porosity data were measured using matrix-cup helium porosimeter for grain volume evaluation and DEB-200 instrument that follows Archimedes law for bulk volume determination.
Porosity data were measured using matrix-cup helium prosimeter (Heise Gauge type) for grain volume calculation and DEB-200 instrument that follows Archimedes principle for sample bulk volume determination.
NR content in conditioned media was measured using matrix-assisted laser desorption ionization (MALDI) MS. A heavy standard of NR was synthesized from hydrolysis of cyanopyridine in 18O water to produce 18O Nam [27], followed by chemical synthesis of heavy NR from heavy Nam [28].
The mass of the purified peptide was measured using matrix-assisted laser desorption or electrospray ionization mass spectrometry, and was consistent with the calculated mass for the peptide.
Next, the mass of the digested proteins, extracted by C18 beads, was measured using matrix-assisted laser disorption/ionization-time of flight (MALDI-TOF) MS as described previously [ 21, 22].
The performance of neural networks is measured using: confusion matrix, accuracy, precision, and sensitivity of the classification.
Cultures were pulsed with 3[H] thymidine for 4 hours on days 1, 2, 3 or 4, and then harvested (Packard, Meriden, CT); the incorporated radioactivity was then measured using a Matrix 96 β-counter (Packard, Downers Grove, IL).
After 72 h, the cultures were pulsed for 18 h with [H]-thymidine (6.7 Ci/mmol; 1μCi/well) and DNA-incorporated radioactivity was measured using a Matrix 9600 direct beta counter (Packard, Meriden, Connecticut, USA; cpm range from 100 to 30,000).
The molecular weight distributions of the asphaltene samples were measured using a matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
Porosity values were measured using both matrix-cup helium porosimeter (Heise Gauge type) for grain volume calculation and DEB-200 instrument which follows Archimedes principle to determine the sample bulk volume.
