Hydrogen evolution rates of about 700 μmol H2 min-1 mg-1 protein, were measured using a hydrogen electrode in the presence of reduced methylviologen as the electron donor (Table 4).
The concentration of H2O2 was measured using a hydrogen peroxide detection kit supplied by Nanjing Jiancheng Institute of Biological Technology (Nanjing, China).
The tumour blood flow was measured using the hydrogen clearance method (Hori et al, 1991, 1996, 2002) that was originally developed by Aukland et al (1964).
H2O2 production by BM-derived macrophages and wound-derived myeloid (CD11b+) cells was measured using the Hydrogen Peroxide Assay Kit (Abcam) and by following the manufacturer's guidelines for the fluorometric assay.
Finally, the electrochemical performance of the cell is measured using pure humidified hydrogen as fuel.
EMF of the other cell was measured using the pumped hydrogen as a standard gas.
The morphology was analyzed using a field-emission scanning electron microscopy (FE-SEM, SU70: Hitachi), and the carbon content was measured using a carbon, hydrogen, nitrogen, sulfur (CHNS) analyzer (Flash EA 1112: Thermo Electron Corp).
Sulfate concentrations of the influent and effluent were measured using ion chromatography and hydrogen, methane, and carbon dioxide concentrations of the reactor feed, bleed, and recycle gas were measured by gas chromatography as previously described (van Houten et al. 2006).
Briefly, up to 100 μmol/L of H2O2 in PBS were incubated with the cell lysates (2 μg protein/μL) for 30 min. The H2O2 remaining in the solutions was measured using the Amplex Red Hydrogen Peroxide Assay Kit (Invitrogen).
PAA coated CNPs are removed by centrifugation and then redispersed in a solution which was buffered at pH 10. Catalase activity of samples was measured using an Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit obtained from Invitrogen.
