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The incorporation of H2AX tagged with green fluorescent protein (GFP) in chromatin of irradiated and unirradiated cells measured using fluorescence redistribution after photobleaching assay (FRAP) was found to be equally slow, indicating that phosphorylation events occur in chromatin in situ rather than by histone exchange (Svetlova et al. 2007).
The tracer diffusion coefficients of these solutes were measured using fluorescence recovery after photobleaching (FRAP).
In addition, the residual stress in the coating was measured using fluorescence spectroscopy.
Lipid oxidation was determined by measuring lipid hydroperoxides and TBARS of the emulsions during storage, while protein oxidation was measured using fluorescence spectroscopy.
Our approach allowed us to test the relative importance of OM quality versus quantity in driving biomass stocks by incorporating FA composition of zooplankton (for fish), abundance of phytoplankton taxa and DOM structure measured using fluorescence spectroscopy (for zooplankton and bacteria), and different measures of wetland area (for POC and DOC).
The former was measured using fluorescence changes of aqueous insulin/silicone dispersions, while the latter examined changes in turbidity as a function of mixing and silicone oil type; the results were confirmed at two different insulin concentrations and agitation speeds.
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The distribution of HER2 receptor expression on the cell lines was measured using fluorescence-activated cell sorting.
Melting temperatures of the receptor variants were measured using fluorescence-based thermal shift assay and for selected mutants by CD melting experiments.
Fluorescence was measured using fluorescence-activated cell sorter analyses with a FACSCalibur flow cytometer, and data were analyzed using CellQuest software (BD Biosciences, San Jose, CA, USA).
L-Lactate and fructose concentrations in the 60-minute serum samples were measured using fluorescence-based assays (both Cayman Chemical Company, Ann Arbor, MI, USA) according to the manufacturer's instructions.
The mean fluorescence intensity of the isolated glomeruli (600 glomeruli from six rats in each group) was analysed using TCS-NT system software (Leica-Microsystems). Intracellular ROS levels were measured using fluorescence-activated cell sorting [ 16].
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