Expression/Ab binding was measured using flow cytometry.
In all experiments Ab staining was measured using flow cytometry.
In all experiments Ab binding was measured using flow cytometry and respective isotype controls were subtracted.
To address this, DU145 cells depleted for PTEN were irradiated with 5 Gy and G2-phase cells were measured using flow cytometry.
Sperm motility was assessed using CASA, and PMAI, HMMP, and % DFI were measured using flow cytometry.
(d) Cryopreserved bone marrow cells isolated from a neonatal calf were measured using flow cytometry to evaluate MHC class I and Integrin beta-1 expression of cells with low and high BNP Ab binding.
After 24 h, cells were transfected with I-SceI expressing vector to induce DSB and the percentage of GFP-positive (GFP+) cells was measured using flow cytometry as an indicator of HR efficiency.
The quantitative analyses were measured using flow cytometry.
The distribution of the cells in the different phases was also measured using flow cytometry.
Additionally, they have found good correlations between TSD and DNA degradation measured using flow cytometry.
Finally, apoptotic proportion of the treated cells was measured using flow cytometry (FACSCalibur, Becton Dickinson).
