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Luciferase activity was measured using dual luciferase reporter assays.
After 48 h, the luciferase activity was measured using dual luciferase reporter assays.
Cytosolic pH was measured using dual emission fluorescent dye, SNARF-1 (Invitrogen).
Body composition was measured using dual energy x-ray absorptiometry (DEXA) scans (GE Lunar Prodigy, Fairfield, CT).
These promoter reporter plasmids along with pRLTK (renilla luciferase vector) were transfected into RKO cells and luciferase activity was measured using Dual luciferase assay kit (Promega).
After washing, peroxidase substrate was added for 15 min. The peroxidase reaction was stopped by adding 100 ml 2.5N sulfuric acid, and absorbance was measured using dual wavelengths of 450 and 595 nm.
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Bone mineral density of the lumbar spine and proximal femur was measured using dual-energy X-ray absorptiometry.
VB bone mineral content (BMC), cross-sectional area (CSA), and volumetric bone mineral density (vBMD) of the third lumbar vertebrae were measured using dual-energy X-ray absorptiometry.
The percentage body fat, absolute fat mass, and fat-free mass were measured using dual-energy x-ray absorptiometry (DEXA) (Model DPX-IQ Lunar Corp., Madison, WI).
After 24 hours, the luciferase activities from each testing construct (A1 A3, A4, A5, A5 or A6) and also from the internal control plasmid were measured using dual-luciferase reporter assay system from Promega Inc.
The percentage trunk and whole body fat were measured using dual-energy X-ray absorptiometry (DXA) QDR-15000, HologiCorp.p., Software version 7.10, Waltham, MA) as described elsewhere [27], [28].
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