Estrogen receptor (ER) levels were measured using the dextran-coated charcoal method as previously described [ 21].
BeWO invasive capacity was measured using a Matrigel-coated transwell invasion assay.
Serum estradiol (E2) and total testosterone (TT) levels were measured using a no-extraction coated tube radioimmunoassay (RIA) (Coat-a-Count, Diagnostic Products Corp., Los Angeles, CA, USA).
Film hardness and elastic modulus as measured using nanoindentation on the coated balls met expectations for chromium nitride, and tribological testing of the coated balls in angular contact ball bearings under moderate contact stress levels demonstrated adequate film adhesion for practical use of these coatings in bearing applications.
Cortisol concentration in saliva was measured using a commercially available, coated-tube radioimmunoassay validated for that matrix [ 46].
Serum immunoglobulins and autoantibodies were measured using Immulon 4 plates (Dynatech Laboratories) coated with 2.5 µg anti-mouse IgG, IgM (Sigma), purified ssDNA (Sigma) or dsDNA (cesium chloride-purified KS+-SV2CAT plasmid) in 100 µl 0.01M PBS, pH 7.4 for 18 hours at 4oC.
The film-coated substrates were removed periodically, the concentration of peptide 1 FL in solution was measured using fluorometry, and the film-coated substrates were placed in fresh buffer.
The initial gravimetric discharge capacity of the Si-coated graphite measured using a half cell was 761 mAh/g at a rate of 0.2 C.
The creep modulus and the adhesion forces of F9 mouse embryonic carcinoma cells (wild-type), vinculin knock-out cells (vinculin −/−), and vinculin −/− cells expressing either the vinculin head domain, tail domain, or full-length vinculin (rescue) were measured using magnetic tweezers on fibronectin-coated super-paramagnetic beads.
