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Protein content was measured using a modification of the Lowry method [47].
PPP activity was measured using a modification of the method described by Hothersall et al. (1979).
Depression symptomatology was measured using a modification of the 20-item Centers for Epidemiologic Studies Depression CESDD) scale [ 62].
Erythrocyte adherence to HUVEC was measured using a modification of the method described by Chung et al. (2007).
The phosphodiesterase activity of ATX was measured using a modification of the method of Razzell and Khorana [ 16].
The hydrogen peroxide content of microdialysates was measured using a modification of the method of Lei et al. (1998).
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Intestinal crypt survival was measured using a modification of microcolony assay [25], [26].
The expression of CD107a, MIP-1β, IL-2, TNF-α and IFN-γ were measured using a modification of a previously described method [26], [27].
Plasma ACE activity was measured using a modification of a previously published method [ 23].
Salivary cortisol was measured using a modification of an unextracted radioimmunoassay method (Diasorin) for serum cortisol.
Intracellular ROS levels were measured using a modification of a protocol previously described (Zuin et al, 2008).
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