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Household wealth is measured using a constructed wealth index based on ownership of 25 household durable assets, house and roof material, livestock ownership and water source.
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Men's knowledge of MNH was measured using a single unit, constructed from a weighted composite scale comprising 39 items across five domains of knowledge, with scale units representing a correct answer to a knowledge question.
Mood states were measured using a mood adjective checklist constructed in Japan; its reliability and validity have been confirmed in studies of Japanese undergraduate students.
Reduced levels of p-ERK/c-Jun led to a significant decrease in AP-1 activity, as measured using a collagenase promoter reporter construct.
The relative fluorescent intensity was measured using a ROI (region of interest) constructed using five concentric circles in the selected Z-stacks.
The similarity among the TIR signals was measured using a position weight matrix (PWM) constructed from the TIR signals of known IS elements in the same IS group [ 35], and the profiles of non-TIR IS elements do not include the TIR signals (see Figure 2 (e)).
Transcription Nuclear factor-κB activity was measured using a Nuclear factor-κB reporter construct and confirmed by Western blot analysis for Nuclear factor-κB p65.
Conceivably, such a construct could be measured using a PROM, enabling change over time to be captured.
The questionnaire constructed for the study consisted of five constructs and all the constructs were measured using a 5-point Likert scale (5 – strongly agree; 1 – strongly disagree).
We also detected a 6-fold upregulation of GADD153 promoter activation measured using a GADD153 promoter-driven EGFP reporter construct and flow cytometry (Fig 3B).
As Francis et al. suggested, each construct should be measured using a minimum of three items.
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