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To determine if the observed associations between flavopiridol PK and SLCO1B1 PGx were functionally relevant for flavopiridol disposition, we measured uptake of flavopiridol and flavo-G in cells transfected with SLCO1B1.
We tested this conjecture by assessing pCA diffusion directly: we measured uptake of pCA by uninjected Xenopus laevis oocytes.
DOI: http://dx.doi.org/10.7554/eLife.00829.008 To test if this manipulation also 'restarts' CME in mitotic cells, we next measured uptake of fluorescent transferrin.
There were 11 studies that measured uptake of ANC, seven community mobilization interventions, three financial incentive interventions, and one with both CM and FI interventions.
To examine membrane permeabilization, we exposed NLRP3-proficient and NLRP3-deficient macrophages to GBS pigment (1 μM) or controls for 20 min and measured uptake of a membrane impermeable dye, propidium iodide (see Materials and Methods for details).
To determine nonreceptor-mediated fluid-phase endocytosis, we measured uptake of HRPO at different intervals of time ranging from 5 to 120 min to assess fluid-phase endocytosis in CS-s (KB-3-1) and Cs-r (KB-CP20) cells, and the results are given in Figure 1.
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3H-proline and 35S-sulfate labels were used to measure uptake of collagen and glycosaminoglycan (GAG) components, respectively.
PMA2020 currently operates in eleven countries worldwide and was established in part to measure uptake of contraceptive use in many of the world's most populous countries (http://www.pma2020.org/).
Endocytic activity was assessed by measuring uptake of the fluid phase marker FITC-dextran (MW 4000; Sigma, USA).
To measure uptake of the FITC-tagged peptide, cells were plated at a density of 5,000 10,000 cells per well in a 24-well plate and grown overnight.
The nondestructive nature of PIXE and the ability to simultaneously measure uptake of multiple metals and endogenous elements are unique to this methodology.
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