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FFL activity was measured under basal growth conditions and following 24 h starvation.
MIC was measured under basal expression conditions.
Expression was measured under basal conditions and after stimulation with varying doses of TNF-α.
GOT activity was measured under basal conditions (before surgery) and 1, 3 and 24 h after reperfusion.
It is possible the Bmi1 promoter was weak in transcriptional activity in this study, as only low reporter activity was measured under basal conditions.
For leptin sensitivity, recombinant mouse leptin (R&D Systems) was injected as an intraperitoneal bolus of 40 µg/day for 5 days, with food intake measured under basal conditions.
Similar(48)
ACTH and corticosterone levels measured under stress-free basal conditions were not different between genotypes at the diurnal peak and trough of the circadian rhythm.
The slope of the relationship between SBP and Na+ excretion (PNat pressure-natriuresis relationship, µEq/mmHg per minute) was calculated by plotting the Na+ excretion on the y axis as a function of SBP on the x axis measured both under basal conditions (time 0 = t 0) and after 2 hours of salt infusion (time 120 = t 120), as described elsewhere [10].
Adenylyl cyclase activity was measured either under basal conditions (without stimulation), or in the presence of forskolin (FSK, 1 μM), D1R specific agonist SKF38391 (SKF, 10 μM) or A2AR agonist CGS21680 (CGS, 10 μM).
The basal proteolysis measured under control conditions was comparable in both cell lines and respectively set as the internal reference (white bars).
In both cases secretion was normalized to the basal secretion measured under control conditions in parallel in the same set of experiments.
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