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In addition to blood gas analysis, we measured total calcium and ionized calcium in serum at baseline and after each SLED run.
In addition to blood gas analysis, we measured total calcium and citrate in serum at baseline and after definitive time points for each CVVHD run.
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In order to measure total calcium from both intracellular calcium release and extracellular calcium influx, HBSS containing physiological calcium levels was used.
To measure total calcium release in human T-lymphocyte populations or Jurkat cell lines, 3×10 cells were loaded with the calcium indicators Fluo-3 and Fura Red (Molecular Probes) at the final concentrations of 5 and 10 μM, respectively, in complete RPMI and incubated for 45 min at 37°C.
We calculated albumin adjusted calcium using the formula: adjusted calcium (mmol/l) = measured total Ca (mmol/l) +0.02 (40− serum albumin [g/l]).
The corrected calcium level based on the serum albumin concentrations was calculated as: Corrected calcium (mmol/L) = measured total Ca (mmol/L) + 0.02 (40 - serum albumin [g/L]), where 40 represents the average albumin level in g/L.
We measured total serum calcium without correction for albumin.
Other factors associated with early death were a history of hypocalcaemia, measured as total calcium (p=0.001; OR 4.27; 95% CI 1.67 to 11.15), and airway malacia demonstrated with bronchoscopy (p=0.043; OR 13.375; 95% CI 1.190 to 110.514).
No difference was seen in measuring the total calcium between groups.
Blood (75 μl) was collected to measure plasma total calcium concentrations at 0, 6, 24, 52 and 78 h from treated and control mice.
In this study, tibial and femoral wet weights were measured to reflect bone mass, whereas serum total calcium was measured as one of the bone metabolism markers, serum alkaline phosphatase was measured as biomarker for bone formation, and C-terminal telopeptide of type 1 collagen (1CTP) was measured as biomarker of bone resorption.
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